We performed immunohistochemical staining for nerve growth factor (NGF) and its receptor TrkA in inner ear tissues of the developing rabbit (days 16 and 21 of gestation). We describe examples that we have used to identify and characterize new eukaryotic vectors, with emphasis on the use of pASZ and pAST vectors.Immunohistochemical localization of nerve growth factor and its receptor in the developing rabbit cochlea: a possible role for nerve growth factor in survival and maintenance of spiral ganglion cells.
This criterion should be applicable to any type of eukaryotic expression vector. This criterion consists of the following five steps: the presence of an open reading frame and a poly-adenylation signal sequence following an initiating AUG a poly-A tail the presence of a Kozak consensus sequence in the 3′ untranslated region of the open reading frame the presence of a cytosolic localization signal and a transcription termination sequence. We describe here a widely-applicable cloning criterion for eukaryotic expression vectors.
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